Authors : Pradip M. Tumane1 and Durgesh Wasnik2
Page Nos : 192-199
Description :
The purpose of this study, to evaluate the multiple drug resistance bacteria from
clinical samples from wound infection E-test method. E-test used for determining the
MIC value of antibiotics for the treatment of patient on what concentration of antibiotic
is suitable. Infection continues to be a major complication of wounds with significant
increase in costs, morbidity and potential mortality. Of these 150 samples, 133 samples
showed positive results for bacteria. From 133 wound samples 149 bacteria were
isolated. These bacteria were screened on different biological media for their isolation
and identification. The Gram negative bacteria were isolated were P. aeruginosa
(34.66%), E.coli (27.33%), P. vulgaris (16%), Klebsiella spp (13.33%), P. mirabilis
(6.66%) and Serratia spp. (1.33%). These samples were tested for their antibiotic
susceptibility testing and followed by MIC by using E- test. The MIC value of Piperacillin
/Tazobactam was found to be 12-16mcg/ml. The MIC value of Cefriaxone and Cotrimaxazole
were found to be in the range of 0.25 to 0.064mcg/ml. The most common
wound infection with Cefriaxone and Co-trimaxazole were the most effective antibiotics
for the treatment of resulting infections based on the culture and sensitivity results
toward multidrug resistant Gram-negative.
Keywords
E-test, Gram Negative Bacteria (GNB), Antimicrobial Resistance, Bacterial Isolates,
MDR
